Performance Compensation Management Annotated Bibliography

Denrell, J., 2005, ‘Should we be impressed with High Performance?’, Journal of Management Inquiry, Vol. 14, No. 3, pp. 292-298. This article discusses the common assumption that high performing firms have efficient management and organisational processes thus differentiating themselves from lower performing firms. The article argues that this actually may not be the case and that high performance in firms may actually be the outcome from several variables such as: economic factors; luck; risk taking; random variables and chance events such as larger resource allocation; and/or incompetence such as a result of mistakes. The author strongly emphasises the main focus of the article which is that due to economic and several other†¦show more content†¦In addition the study also provided areas of considerations for organisations looking to better manage the challenge of cultural differences and team based expatriates assignments. Park, E.J. Huber, D.L., 2007, ‘Balanced Scorecards for Performance Management’, The Journal of Nursing Administration, Vol. 37, No. 1, pp. 14-20. This article describes the benefits and concerns of adopting balanced scorecards as a performance management tool to meet organisational missions and strategies. It discusses the areas of: financial, customer, internal business, and learning and growth perspectives and how they relate to balanced scorecards; the principles involved in using balanced scorecards; how balanced scorecards are beneficial for an organisation; the administrative support required; and the outcomes that can be achieved from employing balanced scorecards as a performance management tool. Although the article does however focus predominately on the implementation of balanced scorecards in healthcare organisations, the approach and topics are still beneficial for other organisations and industries to adopt and implement. In particular, organisations will find that working through the suggested steps for implementing a balanced scorecard approach to help to create a balanced view between financial and customer perspectives, as well creating an administrative tool which can assist in increasing quality,Show MoreRelatedMGT411 week2 Annotated Bibliog Essay656 Words   |  3 Pagesï » ¿ Annotated Bibliography Gilbert Juarez MGT 411 NOV 18, 2014 Professor Martha Alcala Annotated Bibliography Article One: McConnell, C. R. (2007). The Healthcare Managers Human Resource Handbook (1st ed.). Retrieved from UOPX Although this was a Human Resource Management book the topics related to organizational function that deals with issues related to people such as compensation, hiring, performance management, organization development, safety, wellness, benefits, employee motivation, communicationRead MoreHrm Annotated Bibliography Essay1174 Words   |  5 PagesHuman Resource Management’s Leadership Development Programs and the Effects on Employee morale: An Annotated Bibliography Allen, D. G., Bryant, P. C., amp; Vardaman, J. M. (2010). Retaining Talent: Replacing Misconceptions with Evidence-Based Strategies. Academy Of Management Perspectives, 24(2), 48-64. doi:10.5465/AMP.2010.51827775 The authors of this article give the misconceptions of employee turnover by systematically breaking down myths that organizations tend to believe cause employeesRead MoreBusiness Ethics Annotated Bibliography2636 Words   |  11 Pages Annotated Bibliography Marvin Wilcox Liberty University Biong, H., Nygaard, A., amp; Silkoset, R. (2010). The influence of retail management’s use of social power on corporate ethical values, employee commitment, and performance. Journal of Business Ethics, 97, 341-363.Retrieved from http://search.proquest.com.ezproxy.liberty.edu:2048/docview/821301766 The authors of this business journal explore the recent activities of today’s retail giants and how their ethical behavior affects their brandRead MoreIt Organizational Structure2698 Words   |  11 PagesNina Hart ABSTRACT Human Resource Information Systems are the central technology of Human Resource Management. Most HRIS’s are underutilized. The cost to organizations usually outweighs the advantages of ownership. When utilized properly an effective HRIS can improve the bottom line and provide return on investment. The need for a quality improvement initiative of a Human Resource Information System (HRIS) is crucial to the retention of talent, productivity, and improvement of the bottom lineRead MoreEssay on Sexual Harassment In The Workplace2405 Words   |  10 Pagescondition of employment; submission to or rejection of such conduct by an employee is used as a basis for employment decisions affecting the employee; or such conduct has the purpose or effect of substantially interfering with an employee=s work performance or creating an intimidating, hostile, or otherwise offensive work environment. There can be little doubt that sexual harassment is a source of stress found in many of today=s workplaces. The stressful effects of sexual harassment stem primarilyRead MoreEssay about Sodexho HC Consultant Report7105 Words   |  29 PagesBackground 1984 Sodexho HC (â€Å"SHC†) business unit was established under Sodexho group. It is managed through a central office in the Paris area and serves both private and public hospitals in France. Sodexho group is a very successful food and management service provider in the world, its services include a wide range of supports, such as laundry, building maintenance and sterilizing surgical instruments. While Sodexho’s head office is situated in France, some 90% of its business in 2005 was conductedRead MoreGoal Programming3951 Words   |  16 Pagesturn, was and is a powerful nonparametric method for the development of regression functions (e.g. curve fitting) subject to the side constraints. Charnes and Cooper first applied constrained regression in the 1950’s to the analysis of executive compensation. Recognizing that the method could be extended to a more general class of problems – that is, any quantifiable problem having multiple objectives and soft, as well as rigid constraints – Charnes and Cooper later renamed the method Goal ProgrammingRead MoreSexual Harassment in the Workplace4732 Words   |  19 Pagesindividual’s employment; submission to or rejection of such conduct by an individual is used as the basis for employment decisions affecting the individual; or such conduct has the purpose or effect of unreasonably interfering with an individual’s work performance or creating an intimidating, hostile, or offensive working environment. Sexual harassment consists of verbal or physical conduct of a sexual nature, imposed on the basis of sex, by an employee or agent of a recipient that denies limits, providesRead MoreContemporary I ssues in Management Accounting211377 Words   |  846 PagesContemporary Issues in Management Accounting This page intentionally left blank Contemporary Issues in Management Accounting Edited by ALNOOR BHIMANI 1 Great Clarendon Street, Oxford ox2 6dp Oxford University Press is a department of the University of Oxford. It furthers the University’s objective of excellence in research, scholarship, and education by publishing worldwide in Oxford New York Auckland Cape Town Dar es Salaam Hong Kong Karachi Kuala Lumpur Madrid Melbourne MexicoRead MoreMastering Graduate Studies 1e32499 Words   |  130 Pages. . . . . . . . . . . What Makes a Successful Graduate Student. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Transitioning from Student to Scholar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Time Management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Understanding Learning Environments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Conclusion. . . . . . . . . . . . . . . . . . .

Mary Shelleys Frankenstein Essay - 1086 Words

Mary Shelley wrote the book Frankenstein sometime in the 1810s. She was born in London in 1797 (Biography). Her mother was an author of prime literary stock who was trying to encourage women to pursue their ideas and strive to earn the status as equals. The Scientific and Industrial Revolutions that were taking place around Mary Shelley certainly influenced her while she was writing the book. The creation of machines and experiments at the time made people wonder what the limit of human technology was. During the Industrial Revolution machines and technology were being introduced to people that made them worry about the lessening of the impact that humans are making (Industrial). There were protestors at the time that thought these†¦show more content†¦She was trying to instill the message that humanity cannot be replaced. In 1811 there was a revolt by the workers who did not like the way machinery was being implemented into their work (Industrial). They felt that due to th e machines they could not use their skills and that the jobs became degrading. These people thought that they had proven their worth already and did not need these machines to assist them in their jobs, especially since they were now being paid less. A group of these revolts, the Luddite revolts of 1811-1813, took place only a few short years before Mary Shelley wrote Frankenstein. Her husband Percy, was an anti-establishment activist. Neither of them liked the way all jobs were being turned into factory jobs. In the book Victor makes a female companion for the monster but destroys it before it becomes alive. He does this so the monster does not become too human (Burkett). Marriage was created as a human sacrament. One that should not be offered to something that God did not make. This would give the monster too much free will. He chose to have a mate and if it was given to him there would be no difference between him and humans. He had already achieved the intellect by reading boo ks but he could not be given the opportunity to fully experience human emotions. The thought of the horrible things the monster could achieve together scared Victor enough for him to destroyShow MoreRelatedMary Shelleys Frankenstein1689 Words   |  7 PagesGreat Expectations Fathers and Son, Frankenstein. The novel I have chosen to discuss is Frankenstein. Written in 1818 by Mary Shelley, Frankenstein is classified as a gothic novel, however, Shelly uses both realist and non-realist techniques. I will be looking at her reasons for writing the novel and what influenced her, as well as the realist and non-realist techniques used. I will be looking at some of the contemporary social issues that affected Shelley’s life at the time she wrote her novelRead MoreMary Shelleys Frankenstein1179 Words   |  5 Pagesbecome determined to perfect at what they do. They eventually become tragically doomed through creating their own individual moral codes by struggling with their internal battles within their minds. Mary Shelley presents us the first persona of a romantic hero through Victor Frankenstein in her book Frankenstein. Shelley fabricates Victor as the main narrator throughout the book, along with Captain Walton and the creature, which Victor creates. Another hero during the Romant ic era is the Ancient MarinerRead MoreMary Shelleys Frankenstein Feminism1429 Words   |  6 PagesRobert Youshock Prof. Matthew Gerber HIST 1012 10/19/18 Mary Shelley’s Frankenstein: Feminism before it was mainstream? Writing a paper on the topic of Frankenstein days before Halloween might give you the wrong idea- lets clear something up straight away Frankenstein is the doctor not the monster and the monster doesn’t have a name (which we later learn is mildly important to the story). You see, Mary Shelley’s Frankenstein is arguably a story of creation, murder, love, and learning amongst manyRead More Mary Shelleys Frankenstein Essay929 Words   |  4 PagesMary Shelleys Frankenstein The characterization of Victor’s creature, the monster, in the movie although somewhat dramatically different from Mary Shelley’s portrayal in the novel Frankenstein also had its similarities. Shelley’s views of the monster were to make him seem like a human being, while the movie made the monster out to be a hideous creation. The creature’s appearance and personality are two aspects that differ between the novel and movie while his intellectual and tender sidesRead More Mary Shelleys Frankenstein Essay1312 Words   |  6 PagesMary Shelleys Frankenstein In order to illustrate the main theme of her novel â€Å"Frankenstein†, Mary Shelly draws strongly on the myth of Prometheus, as the subtitle The Modern Prometheus indicates. Maurice Hindle, in his critical study of the novel, suggests, â€Å"the primary theme of Frankenstein is what happens to human sympathies and relationships when men seek obsessively to satisfy their Promethean longings to â€Å"conquer the unknown† - supposedly in the service of their fellow-humans†. ThisRead More Mary Shelleys Frankenstein Essay1622 Words   |  7 PagesMary Shelleys Frankenstein Nineteen-year-old Mary Shelley didn’t know when she began it that her â€Å"ghost story† would become an enduring part of classic literature. Frankenstein is an admirable work simply for its captivating plot. To the careful reader, however, Shelley’s tale offers complex insights into human experience. The reader identifies with all of the major characters and is left to heed or ignore the cautions that their situations provide. Shelley uses the second person narrativeRead MoreEssay on Mary Shelleys Frankenstein1643 Words   |  7 PagesMary Shelleys Frankenstein In 1818 a novel was written that tingled people’s minds and thrilled literary critics alike. Frankenstein was an instant success and sold more copies than any book had before. The immediate success of the book can be attributed to the spine-tingling horror of the plot, and the strong embedded ethical message. Although her name did not come originally attached to the text, Mary Shelley had written a masterpiece that would live on for centuries. Read MoreEvil in Mary Shelleys Frankenstein1462 Words   |  6 PagesMary Shelleys Frankenstein is very much a commentary on the Enlightenment and its failure to tame the human condition through reason. The human condition can be defined as the unique features which mold a human being. The creature is undoubtedly a victim of this predicament. He grapples with the meaning of life, the search for gratification, the sense of curiosity, the inevitability of isolation, and the awareness of the inescapability of death. These qualities and his ceaseless stalking of hisRead MoreMary Shelleys Frankenstein Essay846 Words   |  4 Pages Shelley’s Frankenstein does an excellent job at demonstrating the ideas and accomplishments of the enlightenment period. Shelly expresses these ideas and thoughts through the character of Victor Frankenstein who is an aspiring scientist seeking an intellectual challenge. Victor Frankenstein live s his hometown of Geneva and leaves in quest of a va lued education in Ingolstadt. When Victor arrives at college he is lonely and finds himself in a new world in which he lives by himself. He than meetsRead More Mary Shelleys Frankenstein Essay753 Words   |  4 PagesMary Shelleys Frankenstein An outsider is someone who is not a member of a particular circle or group of people He/She is isolated (separated) from other people and regarded as being different such as people looking, dressing, acting or talk differently. Outsiders have always been around and always will exist! Because society (i.e. - those who are not outsiders) like someone to pick on to make themselves feel better or superior. Outsiders are treated in various ways, sometimes people

Evaluation of Genetic Diversity of Golden Apple Snail, Pomacea Canaliculata Free Essays

ABSTRACT Genetics is a trend these days especially that, DNA barcoding has been developed. DNA barcoding is an important tool in categorizing the taxa of different species and it tells so much about the species’ traits, including genetic diversity. The Pomacea canaliculata was introduced in different parts of Asia and had been an invasive species and a pest in different ecosystems ever since the introduction. We will write a custom essay sample on Evaluation of Genetic Diversity of Golden Apple Snail, Pomacea Canaliculata or any similar topic only for you Order Now In understanding this species of snails, samples were collected, DNA’s were extracted, undergone PCR and electrophoresis, and was sequenced and analyzed. The analysis was qualitative In the Philippines while quantitative in China. In the Philippines, the cytochrome oxidase subunit 1 (COI) genes was used and compared among the species collected and when it was sequenced, it showed differences due to localized gap, mismatch and non-correspondence of bases. And in China, the diversity was analyzed by means of Nei’s gene diversity, Shannon’s information index, percentage of polymorphic bands (PPB) an AMOVA anlysis. Both the quantitative and qualitative showed that there was diversification within and among the populations of these snails. INTRODUCTION Genetics is the study of the genes, and the heredity and variation of individuals. Understanding the genome, which is the complete set of chromosomes or the entire genotype of an individual, is important because it helps in the taxonomy of species, especially nowadays wherein advancements in science needs more specific information, and that basing on morphological features is not enough. Life is specified by genomes which contain all the biological information which is encoded in its deoxyribonucleic acid (DNA) and divided into units or the genes. The genes are the blueprint for life because it is the particulate determiner of hereditary traits. Hence, DNA barcoding became a trend for scientists and researchers for the understanding of the different variations in the traits of different organisms. The golden apple snails (Pomacea canaliculata) originated from the South America, Central America, the West Indies and the Southern USA (Pain 1972) and was spread in the past decades to the different parts of Southeast Asia, namely Philippines, China, Thailand, Cambodia, Hong Kong, Indonesia and Japan. The introduction of the P. canaliculata without prior studies caused damages to the different plants and it became an invasive species which resulted to becoming pests to humans and competitors to other local snails, example of which are those from the genus Pili. The P. canaliculata was observed to have different growth and reproduction in different parts of Asia, together with their external characteristics due to the different habitats and environmental conditions (Keawjam, 1986 and 1987), therefore there is the possibility to misidentify two sympatric species as one. On the other hand allopatric populations inhabiting different habitats may show ecomorphological variations and questionable species status and it was also suggested that the golden apple snails had high adaptability hence it was easier for them to form new populations (Dong et al. 2011). The understanding of the genomes of different species is a trend for scientists these days but the information about the different mollusks is still limited. The basic information on the number of species and/or population is of help for conservation programs (Carvalho and Hauser, 1994) and for building appropriate management schemes. In contribution, the studies aims are to evaluate the genetic diversity of the golden apple snail population in Asia, namely, Philippines and China via molecularly characterizing the P. canaliculata and to find different ways of analyzing the gathered data from the sequenced DNA of the said species. REVIEW OF RELATED LITERATURE Genetic diversity (Reed 2005) The significance of genetic diversity arose from two necessities: genetic diversity is required for populations to evolve in response to environmental changes and heterozygosity levels are linked directly to reduce population fitness via inbreeding depression. The amount of genetic variation a population contains is predicted to correlate with current fitness and, in the case of heritabilities (which can remain high or even increase despite severe reductions in population size) with evolutionary potential. This correlation between fitness and levels of genetic variation, however, may be weak or nonexistent due to the neutrality of molecular markers used in estimating heterozygosity, nonadditive genetic variation and the purging of deleterious alleles because of increased selection against homozygotes. There is a body of literature that suggests that allozyme heterozygosity is a good measure of population fitness and adaptive potential. Others caution though that such molecular genetic data generally reflect only a small portion of genome and thus may not be indicator of adaptive genetic differences. But molecular markers may be useful for assessing the extent of genetic drift. Moreover, deleterious alleles, in mutation-selection balance, are responsible for at least half of the genetic variation in fitness. Selection has the tendency to purge the population of the deleterious recessive alleles which in theory creates inbred populations with a higher fitness than their outbreed progenitor. In other words, inbred populations with less genetic diversity would have higher fitness if the population is not kept small enough for a long enough to allow the fixation of deleterious alleles to occur. Fitness and future adaptability are reduced in smaller populations of plants and animals due to drift and inbreeding depression. Commonly used surrogates for fitness such as heritabilities, heterozygosity, and population size, significantly correlate with fitness and explain 15-20 % of the variation in fitness. Correlations suggest that many populations have reduced fitness as a result of inbreeding depression and genetic drift. There is much fuss and concern thus, over genetic variation because of the fact that endangered species typically have lower levels of heterozygosity and the loss of adaptive genetic variation and inbreeding depression puts wildlife populations at an increased risk of extinction. Finally, this increase occurs as a result of the reduction of productive fitness because of inbreeding depression or due to the failure of tracking the change in abiotic and biotic environment of the population as a result of the loss of genetic variation through drift. DNA barcoding (Moritz Cicero 2004) At the very core, the purpose of DNA barcoding is for large scale screening of one or a few reference genes in order to assign unknown individuals to species and enhance discovery of new species. In the hope of developing a comprehensive database of sequences that will serve as a comparison tool to sequences from sampled individuals, proponents used DNA barcoding. There is, however, nothing new with DNA barcoding as it is an offshoot of the use molecular markers for the very same purpose except, in DNA barcoding, there is an increased scale and proposed standardization. The selection of one or more reference genes characterizes standardization, with regards to microbial community and in stimulating large scale phylogenetic analyses if of proven value, though whether or not one gene fits all remain to be a question. Presently, most methods of DNA barcoding are tree-based and can fall into two broadly defined classes. One class is the distance-based, wherein it is based on the degree of DNA sequence variation within and between species. This kind of approach converts DNA sequences into genetic distances and then uses these distances to establish identification schemes. It further defines a similarity threshold below which a DNA barcode is assigned to a known or a new species. There is also the mention by several authors of a â€Å"barcoding gap†, a distance-gap between intra- and interspecific sequences, for species identification. However, the distance-based approach seems to be ill suited as a general means for species identification and the discovery of new species. One reason is that substitution rates of mitochondrion DNA vary between and within species and between different groups of species. The varied substitution rates can result in broad overlaps of intra- and interspecific distances, and hinder the accurate assignment of query sequences. Another class which is the monophyly-based requires the recovery of species as discrete clades (monophyly) on a phylogenetic tree and is used to assign unknown taxa to a known or new species. Similarly, some issues complicate the use of monophyly in a barcoding framework. For example, the long-recognized problem of incomplete lineage sorting will yield gene genealogies that may differ in topology from locus to locus. The recently divergent taxa may not be reciprocally monophyletic due to lack of time needed to coalesce. In addition, the gene trees are not necessarily congruent with species trees, and the monophyly, while a discrete criterion is arbitrary with respect to taxonomic level. Moreover, there is a recently applied new technique that has been proposed as an alternative to tree-based approaches for DNA barcoding, the so called character-based DNA barcode method, which is based on the fundamental concept that members of a given taxonomic group share attributes that are absent from comparable groups. It is the kind of method that characterizes species through a unique combination of diagnostic characters rather than genetic distances. The four standard nucleotides (A,T,C,G) if found in fixed states in one species can be used as diagnostics for identifying that species. This way, species boundaries can be defined by a diagnostic set of characters which can be increased to any level of resolution by applying multiple genes. Presently, character-based DNA barcode method has been proved useful for species identification and discovery of several taxa. In the view that single-gene sequence should be the primary identifier of species, a contention arises that if that is the case then there’ll be a real need to connect different life history stages and increase the precision and efficiency of field studies involving diverse and difficult-to-identify taxa. Although the DNA barcoding community has put emphasis on the importance of large-scale sequence database within the existing framework and practice of systematics, it should be bore in mind that DNA barcoding is not the primary answer in resolving the tree of life. Furthermore, as much as the term â€Å"DNA barcoding† appealing, it implies, however, that each species has a fixed and invariant characteristic. But this kind of implication renders unrest to the minds of evolutionary biologists. In evaluating thus, the promise and pitfall of DNA barcoding, two areas of application should be distinguished: the molecular diagnostics of individuals relative to described taxa and DNA-led discovery of new species. And although there is little doubt that large-scale and standardized sequencing, when integrated with existing taxonomic practice, can contribute significantly to the challenges of identifying individuals and increasing the rate of discovering biological diversity as presented by this study, the general utility of DNA barcoding still requires further scrutiny. PCR (Moore 2005) In rapidly copying a selected template sequence from a DNA mixture in vitro, PCR offers a wide range of applications such as sequence detection and isolation for research, forensics and species identification through the PCR itself and in combination with other techniques. PCR’s new technique uses flourescent probes to monitor the amounted product at end of every cycle and PCR machines look for the cycle at which the can readily detect flourescence. PCR is also being used to monitor RNA through the addition of reverse transcriptase enzyme at the beginning to generate DNA template. In addition, there are now new applications of PCR like single nucleotide polymorphism detection and screening. Cytochrome Oxydase subunit 1 (COI) (Buhay 2009) COI plays a significant role in documenting biodiversity and remains to be the choice for phylogenetic and phylogeographic studies. COI is a mitochondrial protein-coding gene which is a widely accepted marker for molecular identification across diverse taxa. Mitochondrial DNA (mtDNA) have a relatively fast mutation rate, thus they result in significant differences between species. With respect to this, the mitochondrial cytochrome c oxidase subunit I (COI) gene with ~700bp was proposed to be a potential barcode or marker for molecular identification across various taxa. Furthermore, COI is a protein coding gene that has an open reading frame and in thecase of barcoding, COI can be highly divergent from the actual COI sequences which may cause major problems because species identification is based on sequence similarity. Pomacea canaliculata (Cowie 2002) The Pomacea canaliculata belongs to the family Ampullariidae. Its structure appears to have a slight dimorphism in shape of aperture and operculum. Females have broader mouth and concave operculum while convex in male. In terms of reproduction, oviposition often takes place at night or at early morning or evening about 24 hrs after copulation up to two weeks after mating (occurs three times per week) which occurs anytime of the day or night although there may be some diurnal rhythm. On each oviposition occasion a single clutch is laid of highly variable egg number. Moreover, the interval among successive ovipositions for p. canaliculata has been reported to be about five days and hatching generally takes place about two weeks after oviposition. The P. canaliculata breeds only during summer and grows into maturity in less than two months. P. canaliculata is said to be prolific and hence has rapid succession of generations which leads to rapid population expansion. They relatively inhabit still water and in water temperatures above 32 degree Celsius, it has been observed that the mortality of p. canaliculata is high. Whereas in low temperature p. canaliculata can survive 15-20 days at 0 degree Celsius, 2 days at -3 degree Celsius but only 6 hrs at -6 degree Celsius. And it is sufficiently tolerant of sea water to survive long enough to be carried by currents from one stream mouth to another, thereby expanding its distribution. P. canaliculata shows preferences among food plants. Its rate of growth has a direct correlation with its feeding on the preferred plant. Moreover, it is able to detect its food plants from some distance using chemical cues in the water. P. canaliculata, however, appears to be relatively generalist and indiscriminate that it is viewed to be particularly voracious compared to other Ampullariids. METHODOLOGY Sampling Snail samples were identified and collected from 2 countries in Asia, specifically in the Philippines and in China, where the P. canaliculata was introduced. In the Philippines; Los Banos (Dong et al. 011, p. 1778), 2 barangays in Tarlac (Brgy. Cabayaoasan, Paniqui and Brgy. Pance, Ramos) and Iloilo (Chichoco Patdu 2012, p13), 44 snail samples were collected. And in China, specifically from Yuyao and Taizhou in Zhejiang province, Fuzhou in Fujian province, Guangzhou in Guangdong province, Nanning in Guangxi province, Kunming in Yunnan province, wherein a total of 120 samples were identified with the conserved sequence by Ma tsukura et al. (2008) and Pan et al. (2009) and then was collected (Dong et al. 2011, p. 1778). The snails were then stored, either by wrapping in paper, freezing or preserving it in ethanol, and brought into the respective labs in each country for the next steps; DNA extraction, PCR, electrophoresis and sequencing. DNA extraction The two studies used the phenol-chloroform method (Bergallo et al. 2006) with an alternative of the Qiagen’s Dneasy extraction kit for China. The DNA concentration was determined spectrophotometrically and adjusted by a mini-gel method (Maniatis et al. , 1982) when the extracted DNA was enough, it was stored at 4oC to -20oC until needed. PCR and Electrophoresis The PCR method was basically done by choosing the right primers that will yield clearly reproduced bands and they tested the proper amounts and amplification effects of the components of PCR, which were the Mg2+, dNTP’s, DNA templates and polymerase, and the primers. After the mixture of the components and the DNA extracted, it was carried out in the thermocycler programmed for pre-denaturing at 94 °C for 3 min, followed by 26 cycles of 94 °C for 10-30s, 36-52 °C for 30-45s, extension of 65-72 °C for 60-90s, and the final extension for 5-7mins at 72 °C for final extension with 38-48 cycles. After which, the amplified products together with negative controls were run in electrophoresis to be separated and tested for contaminations, respectively. The products were then purified later on with the respective kits present in each lab. In the Philippines, the reaction was done with 2? L MgCl2, 5? L PCR buffer, 1? L dNTP, 2. 5 ? L of the primers, which were the LCO1490 and HCO2198, distilled H2O with 22. 75 ? L, 0. 25 Taq, and 10 ? L Q-buffer. The electrophoresis was done after the ethidium bromide staining (Maniatis et al. , 1982), analyzed through 1. % agarose gels and visualized under a transilluminator. In China, they made use of the ISSR-PCR analysis where they got four primers, which produced clearly reproduced bands, out of the 90 that was screened from the University of British Columbia’s primer set and the reactions were done with a volume of 20 ? l, containing 0. 2 mM of each dNTP, 1. 5 mM MgCl2, 0. 5 ? M primers, 1 U Taq polymerase and 10 ng DNA template, and also with the determination of the optimal reaction system of ISSR for P. canaliculata (Dong et al. 2011, p. 1779). The products’ sizes after the amplification was estimated using DNA marker DL2000 and then was run in electrophoresis, which was done on 6% polyacrylamide gels, visualized by silver staining and then photographed (Li et al. , 2009). Sequencing/ Data analysis Chichioco and Patdu (2012) sent the DNA samples to the First Base Laboratory in Singapore for sequencing and the results were sent back to the DNA barcoding Laboratory after a week. The COI sequences were aligned in the BLAST, specifically the STADEN package version 1. 5. 3 and Bioedit Sequence Alignment Editor version 7. 0. 9. 0. Aside from the sequences sampled, other sequences and their haplotypes from the GenBank were also compared and matched. In Dong’s (2011) research, he made use of the RAPD fragments by labeling them into binary matrices, used them to get the similarity index, Sxy = 2nxy / nx+ ny, where nx and ny represent the number of RAPD bands in individuals x and y, and nxy represents the number of shared bands between individuals, as stated by Nei and Li (1979), then averaging it across all the possible comparisons between individuals within a geographic sample to get the within samples similarity (Si). Between sample similarity corrected by within sample similarity Si and Sj of geographic samples i and j, respectively) is also calculated between pairs of individuals across samples i and j using the equation; S’ij = 1 + Sij – (Si and Sj)/2. Genetic distance between paired samples was then calculated as D’ij = 1- S’ij (Lynch, 1990). RESULTS AND DISCUSSION In the Philippines (Chichioco Patdu 2012, p. 18-31) The collected samples from Brgy. Cabayaoasan were found in the elevated parts of a rice paddy, specifically, it was a muddy substrate with decaying leaves from the rice plants and surrounding trees while those that was found in Brgy. Pance was in the muddy bottom of the shallow fish pond in the roots of water lilies and grasses. The samples from the two barangays in Tarlac and Iloilo had relatively different colors and sizes. Those that were collected from Brgy. Cabayaoasan had the largest size and they are colored black while those in Brgy. Pance had brown in color and still, those in Iloilo had very small sized specimens and some of the specimens can be mistaken as Pila conica snails if not examined properly. Primers affect the amplification success greatly, since according to Hajibabaei (2005) a 95% success is necessary for barcoding. The primers LCO1490 (SENSE) and HCO2198 are generally used for the amplification of forward and reverse fragments from COI genes. The DNA samples were subjected to the PCR and agarose gel electrophoresis (AGE), and they produced single discrete bands that suggest that the fragments were homogenous and start and end at the same point (Reece 2004). The bands that were brighter and distinct are more appropriate for sequencing because it means that the DNA fragments were well amplified. The best DNA’s were chosen and forwarded to the First Base Laboratory in Singapore for sequencing. At the return of the results, other sequenced DNA barcodes were also collected and was aligned and compared with the Basic Local Alignment Search Tool (BLAST) database. Fig 1. Alignment of the COI gene sequences of the Pomacea canaliculata (CPT1-5 from Brgy. Cabayaoasan, PRT 7,9,10 from Brgy. Pance, IICK IIPC1,3 from Iloilo) from the Philippines using Bioedit Sequence Alignment and ClustalW multiple Alignment (Chichioco Patdu 2012, p. 26) By aligning the sequenced data, it can be seen that there are both similarities and differences among the genetic make-up of the samples. The differences are due to localized gap, mismatch and non-correspondence of bases along the COI fragments as pointed out in fig. 2 Fig 2. Comparisons of the COI sequences of the P. canaliculata samples from 35bp- 120bp (Chichioco Patdu 2012, p. 28) As emphasized in fig. 2, on the 55bp-58bp, a sequence from CPT1 was observed having (5’-AATT-3’) while all the others have (5’-GGTA-3’). Even though this is a noticeable difference and could have been caused by mutation or variation, the difference is still low enough and less that 1% difference to be considered significant. But on the other hand, the PCa1 sample had 36 different base pairs compared with the others, which was 5. 5% difference and is high enough and can be considered significant since it is ;4% divergent(Meyer and Paulay 2005). Then with a ~98% confidence, it could be said that PCa1 is from an independent evolutionary lineage and might indicate a divergence within or outside its population or might have occurred due to relationships and interactions among the other species. The introduction of the P. canaliculata to different places may have an effect on its intra- and interpopulation and might be why it has various genetic sequences although it goes against the theory that introduced species becomes a founding population in a new habitat thus they have a limited gene pool and as a consequence genetic drift, which removes variability since it affects all genes, and bottleneck might occur, which reduces the new species to have a reduced genetic diversity. To observe the genetic variability, the sequences collected were compared with those from GenBank with the use of the BLAST software. As a result from 81 COI barcodes and 55 haplotypes, the samples collected showed 99% and 100% similarities with the different haplotypes thus it showed that the species has a high diversity within the populations. The phylogeography within and among the species does not apply on the P. analiculata since intra- and interpopulation diversity was observed which was shown by the multiple introduction throughout the Philippines, hence the different times of the introduction contributed more to its diversity and it coincides with the migrant pool model that says that the introduced population acquires more genetic variability because of the multiple sources of genetically divergent populations as compared to that of the local species (Slatkin 1997, Sakal et al 2001). In China (Dong et al 2010) The chosen primers an average of 124. bands, since they generated a total of 498 bands, which ranged from 150-2500bp and qualifies them for barcoding, as seen in table 1. Among the 140 individuals, 435 bands were polymorphic which was different for each primer. In table 2, Nei’s gene diversity (H) varied between 0. 2612 and 0. 3340, with an average of 0. 3044, and arranged in a descending order the populations, LB ; KM ; NN ; FZ ; TZ ; GZ ; YY while the Shannon’s information index (I) ranged from 0. 3910 to 0. 4856, with an average of 0. 4499. At the species level, the values of Nei’s and Shannon’s showed the same trend as that of PPB. AMOVA analysis showed that there are highly significant (P ; 0. 001) genetic differences among the seven populations of P. canaliculata. The genetic diversity was mostly due to the differences within the population (92. 76%) while the rest was due to among populations. The analysis tells the same as that of the Nei’s and Shannon’s information, which says that there was a relatively high level of genetic differentiation among populations. CONCLUSION Genetics of different species are studied by means of DNA barcoding, mostly of the COI gene in the mitochondria, to know the taxon of a species and to understand their trends and characteristics not only morphologically but also genetically. The diversity of a species can also be tested by means of DNA barcoding as seen in the study of the Pomacea canaliculata. The P. canaliculata was introduced in Asia for agricultural purposes and was seen for its benefits but not its drawbacks, which later on resulted to it being invasive and a pest for both humans and other species. To understand the P. canaliculata further, its diversity was studied by means of DNA barcoding and was analyzed both qualitatively and quantitatively in the Philippines and China, respectively. Both the analysis showed the same outcome, wherein the results showed high levels of genetic diversity among populations. Because invasive species tends to give a negative feedback to those species in the local area, it is important to understand these alien species and to know how diverse they are so that proper management of these species could be done. REFERENCES Barker, G. M. 2002. Molluscs as Crop Pests. UK: CABI Publishing. (pp. 147-182) Buhay, Jennifer E. 2009. ‘‘COI-like’’ Sequences are Becoming Problematic in Molecular Systematic and DNA Barcoding Studies. Journal of Crustacean Biology 29(1): 96–110 Burns, George W. , Bottino, Paul J. 1989. The science of genetics 6th ed. New York: Macmillan Publishing Company. Chichioco, Monique Faye Patdu, Edel Marie. 2012. DNA barcoding of Pomacea canaliculata and Pila conica using cytochrome c oxidase 1 (CO1) and small subunit (ssu) ribosomal (r) RNA barcodes (Undergraduate thesis). Main Library, University of the Philippines Baguio, Baguio City. Cowie, R. H. 2002. Apple snails (Ampullariidae) as agricultural pests: their biology, impacts and management. In: Molluscs as Crop Pests (ed. G. M. Barker), p. 145-192. CABI Publishing, Wallingford. Dodson, Edward O. 1956. Genetics: The Modern Science of Heredity. Philadelphia: W. B. Saunders Company. Dong, S. , Shentu, X. , Pan, Y. , Yu, X. , Wang, H. 2011. Evaluation of genetic diversity in the golden apple snail, Pomacea canaliculata (Lamarck), from different geographical populations in China by inter simple sequence repeat (ISSR). African Journal of Biotechnology Vol. 10(10), 1777-1783. Mendoza, Monica P Serra, Karen Mae S. 2011. Mitochondrial DNA barcoding of Pomacea canaliculata and Pila conica (Undergraduate thesis). Main Library, University of the Philippines Baguio, Baguio City. Moore, Pete. 2005. PCR: Replicating Success. Nature 435, 235-238. Moritz, C. , and Cicero,C.. 2004. DNA barcoding: promise and pitfalls. PLoS Biology 2(10), e354, 1529-1531. Reed, D. H. , Frankham, R. 2005. Correlation between Fitness and Genetic Diversity. Conservation Biology. Conservation Biology 17(1), 230-237. Wang, Xu-Mei. 2010. Optimization of DNA isolation, ISSR-PCR system and primers screening of genuine species of rhubarb, an important herbal medicine in China. Journal of Medicinal Plants Research 4(10), 904-908. Zou, S. , Li, Q. , Kong, L. , Yu, H. , Zheng, X. 2011. Comparing the Usefulness of Distance, Monophyly and Character-Based DNA Barcoding Methods in Species Identification: A Case Study of Neogastropoda. PLoS ONE 4(10), e26619. How to cite Evaluation of Genetic Diversity of Golden Apple Snail, Pomacea Canaliculata, Essay examples

Prostitution the uncontrolalble Vise misc Essay Example For Students

Prostitution the uncontrolalble Vise misc Essay There are women who search for love, and there are those that search for money. Today, the term woman simply denotes ones sex. It does not define her character, morals and values, or even her profession. However, this was not always the case. At the end of the nineteenth century and beginning of the twentieth century, during the Progressive Era, there was a drive for reform. Various social problems became targets for investigation and intervention: child labour, juvenile delinquency, corruption in city government and police departments, and prostitution. These things were newly discovered social problems; the only differences during this period were the new assumptions, strategies, and expectations of a broad organization of activists. Progressive reform actively decided to take more of a role in regulating the social welfare of its citizens, and those private and public spheres of activity could not be disentangled. Prostitution was an issue that underscored the relationship between home life and street life, wages of sin and low wages of women workers, double sexual standards and transmission of venereal disease. The late nineteenth century response to prostitution revealed the competing ideologies within Progressive reform activity over social justice and social control. Most attempts to deal with prostitution have consisted almost exclusively of more or less vigorous attempts to suppress it altogether by forcing the closing of brothels, and by increased police activities against individual prostitutes and against those individual places, such as taverns, where prostitutes frequently solicit. This paper seeks to prove that the reformers were unable to stamp out prostitution during the end of the nineteenth century and the beginning of the twentieth century for a variety of factors. First, I will look at why women in the late nineteenth, and early twentieth century became prostitutes. The gender differences between sex roles will be analyzed in relation to prostitution. Finally, the various failed attempts to abolish prostitution will be discussed. Legally prostitution is often defined as the hiring out of the body for sexual intercourse. Some say that the exchange of money does not need to take place. Albert Ellis, one well-known sexologist and author would define prostitution as, A woman or a man engaging in sexual relations for non-sexual and non-amotive considerations. This definition would therefore include girls who trade their sexual favors for food, entertainment or other gifts. Each individual may have different views as to what a prostitute is or how they feel about them. During the late nineteenth century and early twentieth century reformers, for example, wanted to eradicate prostitution. It was looked at as the cause of all evil and poverty, among other things. But, it was over the place, girls supplying their bodies for the males high compulsion to satisfy their sexual desires. Canadas industrial development equipped many women with outlet for their skills and energies in addition to the home and other work places. With all the improvements in transportation and communications, growth of the cities is the availability of new consumer goods provided in an age of national growth. However, with all of this came economic and social tensions. Most Canadians were concerned with the presence of certain ethnic groups, poverty in the cities and an increasing crime rate. With this new found awareness of social problems, came the belief that by identifying and classifying problems the nature of the world could be reformed to insure a moral, civilized society. There are many reasons why one would choose upon a career of prostitution. They range from quick money to language barriers (most girls were foreign born or their parents were foreign born), from curiosity to alcoholism. Most prostitutes are believed to have started at a young age and despite much talk about white slavery, no cases were ever found of a women unwillingly detained in a brothel. Up until about mid way through the twentieth century a large percentage of all the women engaged in prostitution were professional prostitutes, registered or widely known to be such, and often working in brothels. A the 1916s Unemployment Commission had observed, working girls seemed to be unable to stick out jobs for more than a few months, and many were so frivolous and irresponsible that they were justifiably dismissed. .